Project

Part:BBa_M36777:Design

Designed by: Evan Clark   Group: Stanford BIOE44 - S11   (2011-05-03)


DNA damage sensor with weak LexA generator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 782
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 782
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 782
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 782
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 782
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sensor with a weaker LexA generator is another candidate for level matching with the Gemini actuator we will attach to the damage sensor.


Source

LexA gene and promoter from E. coli genome, other parts from registry

References